mouse monoclonal antibodies against her3  (R&D Systems)

Journal: World Journal of Oncology

Article Title: Synergistic Effects of Neratinib in Combination With Palbociclib or Miransertib in Brain Cancer Cells

doi: 10.14740/wjon1873

Figure Lengend Snippet: Surface Expression of Various Growth Factor Receptors in Human Brain Cancer Cell Line (Determined by Flow Cytometry)

Article Snippet: The mouse monoclonal antibodies against HER3 (MAB3481), HER4 (MAB11311), ALK7 (MAB77491) and HGF R/c-MET (MAB3582) were purchased from R&D Systems (Oxford, UK).

Techniques: Expressing, Cytometry, Control, Fluorescence

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Risk of worse breast cancer-specific or distant metastasis-free survival conferred by high protein levels HER3, EGFR, or HER3-EGFR protein expression in univariate Cox models stratified by hospital.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Risk of worse breast cancer-specific or distant metastasis-free survival conferred by high protein levels of HER3, EGFR, or HER3-EGFR in age- and stage-adjusted Cox models stratified by hospital.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques:

Journal: Scientific Reports

Article Title: Combined HER3-EGFR score in triple-negative breast cancer provides prognostic and predictive significance superior to individual biomarkers

doi: 10.1038/s41598-020-59514-1

Figure Lengend Snippet: Predicted causal networks explaining observed gene expression differences based on combined HER3-EGFR protein expression.

Article Snippet: For HER3, antigen retrieval was performed using the Dako Target Retrieval Solution at pH 9 in a 90 °C water bath for 35 min. Then, slides were incubated with monoclonal mouse anti-human HER3 antibody (DAK-H3-IC) at 1:25 for 30 min. For EGFR, antigen retrieval was performed using Proteinase K (Agilent, S3020) for 6 min on a hot rack.

Techniques: Expressing, Activation Assay

Journal: Cancer Cell International

Article Title: Establishment of primary human breast cancer cell lines using “pulsed hypoxia” method and development of metastatic tumor model in immunodeficient mice

doi: 10.1186/s12935-019-0766-5

Figure Lengend Snippet: Expression of HER2, HER3, Vimentin and Ki-67 in primary and cultured cells. a Analysis of surface expression of HER2 and HER3 by flow cytometry with specific antibodies. b Double immunofluorescence staining of Vimentin (green) and Ki-67 (red). Immunocytochemical detection (×40) in cancer and normal human cells

Article Snippet: FITC-conjugated mouse anti-human HER2 monoclonal (#2222020), FITC-conjugated mouse anti-human CD326 monoclonal (#2221020), PE -conjugated mouse anti-human CD324 monoclonal (#2220530), PE -conjugated mouse anti-human CD325 monoclonal (#2354025), allophycocyanin (APC)-conjugated mouse anti human HER3 monoclonal (#2223540), APC-conjugated mouse anti human CD146 monoclonal (#2310060) antibodies were purchased from Sony Biotechnology Inc. (San Jose, CA, USA).

Techniques: Expressing, Cell Culture, Flow Cytometry, Double Immunofluorescence Staining

Journal: Journal of colloid and interface science

Article Title: A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems.

doi: 10.1016/j.jcis.2018.11.035

Figure Lengend Snippet: Figure 6. PSDs of (A) MCAM- and ErbB3-SERS nanotags with 0.1% w/v BSA coatings in

Article Snippet: Human anti-erythroblastic leukemia viral oncogene homolog 3 (ErbB3) and anti-melanoma cell adhesion molecule (MCAM) mouse monoclonal antibodies (MAB3481 and MAB932) were bought from R&D Systems and prepared with 1× PBS.

Techniques:

Journal: BMC Cancer

Article Title: Characterization of primary normal and malignant breast cancer cell and their response to chemotherapy and immunostimulatory agents

doi: 10.1186/s12885-018-4635-8

Figure Lengend Snippet: Expression of biological markers in MCF-7, BC5, BrCCh1, and BN1 cells. a Expression levels of the progesterone receptor (PGR), oestrogen receptor (ER) α and β, and Cyp19 mRNA in primary cell lines according to real-time polymerase chain reaction (PCR) analysis. The expression of specific mRNAs was normalised to the expression level of glyceraldehyde 6-phosphate dehydrogenase (GAPDH) mRNA. The expression levels of PGR, ERα, ERβ, and Cyp19 mRNA in the different cell lines are shown relative to their expression level in MCF-7 cells where it was set equal to 1. Statistical analysis included the results of two independent experiments (mean ± SD). * The difference between the experimental group and the control (MCF-7) group was statistically significant at p < 0.05. b Representative flow cytometry data of human epidermal growth factor receptor HER2(CD340)/HER3 positive cells in BC5 and BrCCh1 cells. Stained cells were gated according to isotype control samples so that these cells were negative to both HER2/HER3 (left bottom quadrant). Cells from right upper quadrant were accounted as HER2/HER3 double-positive cells

Article Snippet: FITC-conjugated mouse anti-human HER2 monoclonal and allophycocyanin (APC)-conjugated mouse anti human HER3 monoclonal (#2223535) antibodies were purchased from Sony Biotechnology Inc. (San Jose, CA, USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Flow Cytometry, Staining